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Welcome to CoLocalizer Support

Built-in Help


Both CoLocalizer Pro for Mac and CoLocalizer for iPad include built-in Help. In CoLocalizer Pro for Mac Help can be accessed under the Help menu. In CoLocalizer for iPad, open image and then tap Settings > Help. Handy tips for both apps are also available.


iCloud Syncing


CoLocalizer images are synchronized automatically across all your devices via and stored in iCloud Drive.  For this functionality to work, you need installed iOS 8 and newer on your iPad(s) and OS X 10.10 (Yosemite) and newer on your Mac(s) and have any version of CoLocalizer for iPad and CoLocalizer Pro 5 and newer for Mac.

CoLocalizer for iPad Terms of Use


CoLocalizer for iPad Terms of Use can be found here.

Import Compatibility


CoLocalizer apps can open RGB files in the most popular file formats, such as TIFF, JPEG,  and PNG. They don't work with gray scale images. The use of lossless TIFF is strongly recommended to ensure the reliability of calculations. CoLocalizer Pro supports proprietary file formats from all major microscope manufacturers and a biological image data standard:

• Zeiss: LSM, TIFF, TIFF Chunky, RGB PLANAR, etc.

• Leica: TIFF.

• Open Microscopy Environment: OME.TIFF.

Export Compatibility

• exports images in various file formats for presentation and publication.

• saves calculations as Excel files that can be processed using Microsoft Excel spreadsheet application.

• saves Text files for reference.

• saves calculations as HTML files for posting on the Internet.

• saves files in PDF format for submission to the publishers and sharing with colleagues.

Cross-Platform Compatibility


The ability of CoLocalizer apps to export PDF, HTML, and Microsoft Excel files makes it possible to present the results of your calculations to others and easily share ideas across computer platforms.


CoLocalizer Pro video tutorials take you through  the steps needed to properly quantify colocalization in your fluorescence microscopy images.



We have prepared a list of questions frequently asked by our users (FAQ). If you have questions, make sure you have checked FAQ first. The possibility is high they are already answered.


Release Notes

Wonder when a particular feature was introduced and what was changed? Read the release notes for CoLocalizer Pro for Mac and CoLocalizer for iPad.



One of the most exciting places on CoLocalization Research Software web site is blog. It contains valuable information about colocalization, press releases, insights into CoLocalizer features, and many other related things.



If you experience unexpected issues with CoLocalizer Pro app, it is possible that its preference file is corrupted. To check it, go to Users>yourUserName>Library>Preferences and locate the com.CRS-CoLocalizer.Pro.plist file. Move this file out of the Preferences folder. Then, start the app. If no issues remain, delete the moved .plist file. It was causing the issues. To access the Library folder, press and hold the Option (Alt) key and click the Go menu in the Finder. You will find the Library folder there.

Contact Support Team


You should not have questions by now, but in the case you still have, feel free to contact us via the form below:

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