Colocalization is a visual phenomenon commonly observed in fluorescence microscopy images, where differently labeled fluorescence molecules appear very close to each other, causing their colors to overlap.
Quantifying colocalization provides statistical evidence for studies that describe the location and possible interactions of fluorescently labeled objects.
What’s CoLocalizer?
CoLocalizer is a Machine Learning-powered application designed for analyzing colocalization. It is available as CoLocalizer Pro for Mac (desktop version) and CoLocalizer for iPad (tablet version):
CoLocalizer Pro for Mac and CoLocalizer for iPad work hand-in-hand and can be used interchangeably.
“Pro” means that the app includes a complete set of features and tools for colocalization analysis. It 1) allows users to open single-channel images and merge them for analysis, 2) calculates all established coefficients, and 3) exports results as images and Excel files.
Yes, you can try CoLocalizer before making a purchase. A free trial can be activated after downloading and installing CoLocalizer Pro on your Mac. The trial is limited to several sessions. However, any data obtained during the trial cannot be used for presentation and publication without purchasing the full license. CoLocalizer for iPad is available as a free download from the Apple App Store, but a subscription must be purchased to analyze custom images.
CoLocalizer Pro for Mac:
CoLocalizer for iPad:
Follow these three steps:
CoLocalizer apps work with RGB images. If your image data is stored in two separate single-channel files, you can combine them into a single RGB image using the merge tool in CoLocalizer. Please note that CoLocalizer does not support grayscale images and will not open and analyze them.
Can I skip the background correction step?
No, it is essential never to skip the background correction step. This can be performed using a Machine Learning model in either CoLocalizer Pro for Mac or CoLocalizer for iPad.
When citing CoLocalizer apps in publications, include the app name, version, company name (CoLocalization Research Software), and the URL (www.colocalizer.com).
CoLocalizer utilizes modern programming technologies exclusive to macOS and iPadOS, making it virtually impossible to create similar versions for Windows/Android without sacrificing functionality.
For further questions, please contact us via the Contact form or use the Visit Support Page menu item under the Help menu in CoLocalizer Pro for Mac.