New in CoLocalizer Pro for Mac

CoLocalizer Pro 7.1.2 (2026.10.15)

🧮 The overlap R (Manders') coefficient could occasionally be incorrect for larger images - fixed.
✅ Improved image import validation: grayscale images are now detected during import and a clear message explains that RGB images are required for colocalization quantification.
💾 When exporting and saving JPEG files, you can now preview the file size based on the selected quality.
📂 Improved support for opening multi-slice .lsm files.
🗂️ When exporting and saving split files, it is now possible to choose either uncompressed or compressed TIFF format options.
🔁 Added channel pair undo support and a mismatch alert in the Colocalization view to warn users that changing the channel pair after the Background Correction step may affect result accuracy.
🎯 Lasso and Polygon ROI selections are now more precise. Crossing selection lines when using Lasso or Polygon now cancels the selection, matching CoLocalizer for iPad’s ROI selection behavior.
🔄 Undo/Redo for ROI selections is now working more reliably.
📐 Enforced minimum ROI size on all shape types.
📦 .colocalizer documents now take up far less space, which makes them save, sync and copy faster.
🚀 Added a modern launch window.
🏗️ More UI elements now use modern SwiftUI.
⚙️ Made various stability and performance enhancements.
⚠️ Now requires macOS 14 (Sonoma) or newer.

CoLocalizer Pro 7.1.1 (2026.05.05)

🧭 Inspector, Background Correction, and Colocalization menu items have been moved into a submenu under Tools > Analysis for better organization.
🔄 The slice panel now opens automatically the first time a multi-slice image is opened.
✨ Optimized image rendering for Retina and High-DPI displays: the Region of Interest (ROI) now respects system scaling, ensuring images are displayed at the correct physical size with maximum sharpness.
♻️ Added a Reset button in ROI Settings to quickly restore the Selection Line Color to its default.
✅ Multi-slice reordering was not persisting across saves - fixed.
🔧 When deleting slices in an image stack, the Inspector did not update the number of remaining slices correctly - fixed.
⚙️ Made various stability and performance enhancements.

CoLocalizer Pro 7.1 (2026.04.02)

🚀 Now uses Metal compute shaders for better performance and efficiency.
✨ 16-bit TIFFs can now be opened and analyzed correctly, with intensity relationships preserved.
🖼️ Rectangular images appeared distorted in square-sized background correction presets - fixed.
🤌 Trackpad zoom is now smoother and more consistent.
⚙️ Made various stability and performance enhancements.

CoLocalizer Pro 7.0.7 (2026.03.03)

CoLocalizer Pro 7.0.6 (2025.12.17)

CoLocalizer Pro 7.0.5 (2025.07.22)

CoLocalizer Pro 7.0.4 (2025.06.26)

CoLocalizer Pro 7.0.2 (2021.08.13)

The redesigned architecture of the ML Super Resolution model significantly decreased the use of system memory: now requires 7.5 times less RAM.

CoLocalizer Pro 7.0.1 (2021.01.19)

CoLocalizer Pro 7.0 (2020.08.04)

Introducing ML Super Resolution. Revolutionary Machine Learning-powered transformation of conventional fluorescence images to images comparable with super resolution.
Now requires macOS 10.13 (High Sierra) or newer.

CoLocalizer Pro 6.1 (2020.05.05)

The prediction accuracy of the Background Correction model has improved to 99%.
When changing channel pairs with Reveal Colocalized Pixels selected, pixels on the image were not updated. Fixed.
It is now possible to export either uncompressed or compressed (LZW, Packbits) TIFF files.
Improved layout of the report files.

CoLocalizer Pro 6.0 (2019.12.21)

Introducing Machine Learning-enhanced background correction. The Deep Learning model provides over 90% prediction accuracy of various background noise levels.
Redesigned Background Correction pane, and updated tooltips.
Histogram channels are now shown more distinctively.
Updated to the latest version of Sparkle update framework.
Updated graphics.