We are often asked about the way CoLocalizer counts pixels. The general answer is that pixels are counted according to the selected pairs of channels taking into consideration color components. This means that if, for example, you've selected the Red-Green pair of channels, Blue pixels will not be included into the count. To count a pixel as Red, it has to have some value of Red and no Green and no Blue. To count a pixel as Green, it has to have some value of Green and no Red and no Blue. To count a pixel as Yellow, it has to have some value of Red and some value of Green and no Blue.
When the image is analyzed according to the Red-Green pair of channels and it contains Blue pixels, the latter will not be included into the count and the resulted pixel count may, therefore, be less than 100%.
We should also mention that in the just released version of CoLocalizer Pro 3, we've changed the way how the background is corrected and now recommend that users correct it using selected pairs of channels, not single channels, as it was before. It makes little sense when correcting background to consider pixels of a third channel that will be excluded from the count. If users want to see pixels of a third channel, they can do it using the Histogram tool.
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